Bioxan® Tocotrienols
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Advanced Vitamin E Innovation for Visible Skin Performance
While tocopherols are widely used in cosmetic formulations, tocotrienols represent the most biologically potent members of the Vitamin E family. Thanks to their unsaturated isoprenoid side chain, tocotrienols exhibit superior membrane mobility, optimized antioxidant distribution, and broader biological activity.
Bioxan® Tocotrienols deliver a next-generation, multi-target dermocosmetic solution, clinically evaluated under dermatological control with robust statistical analysis.
Clinically Demonstrated
In Vitro Performance
The anti-inflammatory activity of Bioxan® Tocotrienols (1%) was assessed in stimulated human keratinocytes by measuring pro-inflammatory cytokine production.
Experimental Design
Keratinocytes (100% confluent) were cultured in DMEM + 10% FBS.
- Negative Control (NCM): non-stimulated cells
- Inflammation Control (IC): cells stimulated with Invitools™ inflammatory agent
Cells were exposed to the inflammatory stimulus ± tocotrienols (1%) for 1.5 h, followed by overnight incubation (≥ triplicates).
Analysis
- Cytokines: IL-1β, IL-6, IL-8 (immunoassay, 450 nm), normalized to viable cells
- Cell viability: MTT assay (570 nm)
Results
Bioxan® Tocotrienols significantly reduced IL-1β, IL-6, and IL-8 levels in stimulated keratinocytes without affecting cell viability, confirming anti-inflammatory activity independent of cytotoxic effects.
In Vivo Performance
56-Day Dermatological Study (n = 22)
Study Design Overview
- Open-label, single-arm clinical study
- 22 female subjects (47–62 years)
- Twice-daily facial application
- Duration: 56 days
- Evaluation time points: D0 (baseline), D28, D56
- Significance level: p < 0.05 (95% confidence interval)
- All measurements performed under standardized environmental conditions
The study evaluated five major skin parameters:
- Hyperpigmentation
- Fine lines
- Elasticity
- Erythema (redness)
- Glycation (AGEs accumulation)
1. Depigmenting Effect – Targeted Dark Spot Reduction
Methodology
- Instrument: Mexameter® MX18
- Parameter: Melanin Index
- Measurements performed:
- Directly on hyperpigmented spot
- On adjacent normal skin (reference area)
- Three consecutive measurements per site
Statistical Analysis
- Spot vs adjacent skin: Wilcoxon signed-rank test (paired, non-parametric)
- Time-point comparisons (D28, D56 vs baseline):
- Linear Mixed-Effects Model (LMM) when normality was met
- Wilcoxon test when assumptions were not met
- Significance threshold: p < 0.05
Results
- At baseline: Dark spots showed 38% higher melanin levels vs surrounding skin (p < 0.05)
- Day 28: 19% reduction in spot melanin
- Day 56: 21% reduction in spot melanin
- Contrast reduced from 38% to 21% (p < 0.05)
- Surrounding skin reduced only 9%, confirming a targeted depigmenting effect
Conclusion: Significant and selective reduction of hyperpigmentation after 56 days.
2. Anti-Glycation Effect – Reduction of AGE Accumulation
Methodology
- Instrument: AGE Reader mu
- Parameter: Skin autofluorescence (quantification of Advanced Glycation End-products)
- Three consecutive measurements per zone
Statistical Analysis
- Continuous variable
- Linear Mixed-Effects Model (LMM)
- Significance set at p < 0.05
Results
- After 56 days: 6% reduction in AGEs vs baseline
Conclusion: Demonstrated protective effect against glycation-related aging mechanisms.
3. Anti-Erythema Effect – Visible Redness Reduction
Methodology
- Instrument: Mexameter® MX18
- Parameter: Hemoglobin Index
- Randomized cheek area
- Three consecutive measurements averaged
Statistical Analysis
- Continuous variable
- Linear Mixed-Effects Model (LMM)
- D28 and D56 compared vs baseline
- p < 0.05
Results
- Day 28: 9% reduction in hemoglobin index
- Day 56: 8% reduction vs baseline
Conclusion: Clinically proven soothing and anti-redness efficacy.
4. Elasticity Improvement – Enhanced Viscoelastic Balance
Methodology
- Instrument: Cutometer® Dual MPA 580
- Parameter: R6 (Uv/Ue ratio)
- Lower R6 values indicate improved elasticity
Statistical Analysis
- Continuous variable
- Linear Mixed-Effects Model (LMM)
- Significant reductions at D28 and D56 (p < 0.05)
Results
- Day 28: 31% decrease in R6
- Day 56: 31% decrease vs baseline
Conclusion: Strong improvement in skin elasticity and biomechanical resilience.
5. Reduction of Fine Lines
Methodology
- Clinical evaluation using a 10-point ordinal photo-clinical grading scale (0–9)
- Standardized photography conditions
- Lower score = fewer visible fine lines
Statistical Analysis
- Ordinal variable
- Cumulative Link Mixed Model (CLMM)
- Comparison: D28 and D56 vs baseline
- Significance: p < 0.05
- Median considered most robust descriptor
Results
- After 56 days:
- 20% median reduction in global fine lines score
- Statistically significant (p < 0.05)
Conclusion: Clinically validated wrinkle-reducing efficacy.
Bioxan® Tocotrienols
Scientifically Substantiated Multi-Target Performance
Bioxan® Tocotrienols demonstrated:
- Dark spot reduction (21%)
- Fine line reduction (20%)
- Elasticity improvement (31%)
- Redness reduction (8–9%)
- Anti-glycation protection (6%)
A High-Value Active for Next-Generation Dermocosmetics
Bioxan® Tocotrienols provide a scientifically validated, multifunctional approach to:
- Anti-aging formulations
- Brightening treatments
- Sensitive skin care
- Urban pollution defense
- Dermocosmetic repair
Backed by instrumental measurements, appropriate statistical modeling, and dermatological supervision, this ingredient offers measurable biological performance translated into visible clinical outcomes.
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Bioxan® Tocotrienols
What are Tocotrienols?
Tocotrienols are a variant form of natural Vitamin E, consisting of eight distinct molecules. These molecules are divided into two categories: tocopherols and tocotrienols. Each category contains four members, known as alpha, beta, delta, and gamma (Jiang et al., 2009).
Bioxan® Tocotrienols: The Next-Level Cosmetic Active for Inflammation and Antioxidant Protection
Tocotrienols are emerging as a highly beneficial active ingredient in the cosmetics industry, with exceptional advantages for skin health. While tocopherols are recognized for their antioxidant properties, tocotrienols offer superior benefits due to their unique anti-inflammatory capabilities. These properties are particularly valuable as consumers increasingly look for solutions for skin inflammation caused by environmental stressors and modern lifestyles.
Investigation of the Anti-Inflammatory Properties of Bioxan Rice Tocotrienol 50 and Bioxan Palm Tocotrienol 50
Chronic inflammation plays a crucial role in skin aging and the onset of inflammatory skin disorders. External stressors, such as ultraviolet (UV) radiation and environmental pollutants, can activate an inflammatory response in keratinocytes, leading to the release of pro-inflammatory cytokines, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8).
To assess the potential protective effects of two tocotrienol-rich fractions—Bioxan® Tocotrienol Rice 50 (Sample 1) and Bioxan Tocotrienol Palm 50 (Sample 2)—a study was conducted using an in vitro model with human keratinocytes.
Experimental Design
Human keratinocytes were cultured as a monolayer until full confluence was achieved. The cells were then exposed to an inflammatory agent (INVITOOLS™) in the presence or absence of the test samples to evaluate their effects on cytokine production. The levels of IL-1β, IL-6, and IL-8 in the cell culture supernatants were quantified after incubation.
Assessment of Cytokine Inhibition
To determine the extent of cytokine suppression, the collected samples were analyzed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Statistical comparisons were conducted using Student’s t-test to evaluate the significance of the observed effects.
Parameters Evaluated
- Inflammatory response: The production levels of IL-1β, IL-6, and IL-8, which are crucial markers of inflammation.
Results
- Sample 1 (Bioxan Tocotrienol Rice 50, 1%) exhibited a significant reduction in IL-8 production, highlighting its potential as an anti-inflammatory agent.
CAPACITY OF PROTECTION AGAINST IL-8 PRODUCTION (pg/ml)
- Sample 2 (Bioxan Tocotrienol Palm 50, 1%) demonstrated a broader inhibitory effect, significantly reducing the synthesis of IL-1β, IL-6, and IL-8, indicating a
CAPACITY OF PROTECTION AGAINST IL-1β PRODUCTION (pg/ml)
CAPACITY OF PROTECTION AGAINST IL-6 PRODUCTION (pg/ml)
CAPACITY OF PROTECTION AGAINST IL-8 PRODUCTION (pg/ml)
Conclusion
The findings suggest that both tocotrienol-based extracts possess anti-inflammatory properties. While Sample 1 effectively decreases IL-8 levels, Sample 2 provides a more extensive reduction in IL-1β, IL-6, and IL-8, positioning it as a strong candidate for mitigating chronic inflammation and delaying premature skin aging.
